4XWF

Crystal structure of the ZMP riboswitch at 1.80 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99995
Spacegroup name	P 43 21 2
Unit cell lengths	40.160, 40.160, 234.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.751 - 1.800
R-factor	0.2137
Rwork	0.210
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xw7
RMSD bond length	0.006
RMSD bond angle	1.201
Data reduction software	MOSFLM
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.260	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.057	0.574
Rpim		0.471
Number of reflections	19001
<I/σ(I)>	2	2
Completeness [%]	99.5	99.3
Redundancy	3.9	3.6
CC(1/2)		0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		303.15	PEG 8K, ammonium acetate, magnesium acetate, cacodylate