4XWF
Crystal structure of the ZMP riboswitch at 1.80 angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99995 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 40.160, 40.160, 234.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.751 - 1.800 |
R-factor | 0.2137 |
Rwork | 0.210 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xw7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.201 |
Data reduction software | MOSFLM |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.260 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.057 | 0.574 |
Rpim | 0.471 | |
Number of reflections | 19001 | |
<I/σ(I)> | 2 | 2 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 3.9 | 3.6 |
CC(1/2) | 0.796 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | PEG 8K, ammonium acetate, magnesium acetate, cacodylate |