4XVR

H-Ras Y137F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-06-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	39.234, 39.234, 157.204
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.978 - 2.031
R-factor	0.2283
Rwork	0.222
R-free	0.28570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctq
RMSD bond length	0.008
RMSD bond angle	1.127
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.000	42.000	2.070
High resolution limit [Å]	2.030	5.510	2.030
Rmerge	0.058	0.037	0.298
Rmeas	0.062	0.040	0.314
Rpim	0.021	0.013	0.097
Total number of observations	89345
Number of reflections	9756
<I/σ(I)>	20.2
Completeness [%]	99.5	99.8	100
Redundancy	9.2	8.1	10.1
CC(1/2)		0.999	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	139mM Ca(OAc)2, 22.6% PEG 3350, 13% stabilization buffer at pH 7.5