4XVP
X-ray structure of bGFP-C / EGFP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.520, 71.550, 179.200 |
| Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
| Resolution | 46.700 - 3.400 |
| R-factor | 0.21327 |
| Rwork | 0.209 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EGFP PDB entry 1JBZ 6-helix motif from alphaRep4 PDB entry 3LTJ |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.442 |
| Data reduction software | XDS (13-Jan-2015) |
| Data scaling software | XSCALE (13-Jan-2015) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.100 | 0.540 |
| Number of reflections | 17746 | |
| <I/σ(I)> | 10.85 | 2.03 |
| Completeness [%] | 96.5 | 80.2 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 50 mM tricine pH 6.9, 25% PEG4K |
