4XVI
Binary complex of human polymerase nu and DNA with the finger domain ajar
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 292.665, 292.665, 110.497 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.655 - 3.100 |
R-factor | 0.2146 |
Rwork | 0.213 |
R-free | 0.23660 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.112 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.150 |
High resolution limit [Å] | 3.100 | 8.360 | 3.100 |
Rmerge | 0.119 | 0.080 | 0.612 |
Total number of observations | 145500 | ||
Number of reflections | 32918 | ||
<I/σ(I)> | 10.2 | ||
Completeness [%] | 99.6 | 98.5 | 98.2 |
Redundancy | 4.4 | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.5 M Ammonium Sulfate, 100 mM MES |