4XVI

Binary complex of human polymerase nu and DNA with the finger domain ajar

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	292.665, 292.665, 110.497
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.655 - 3.100
R-factor	0.2146
Rwork	0.213
R-free	0.23660
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.112
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.150
High resolution limit [Å]	3.100	8.360	3.100
Rmerge	0.119	0.080	0.612
Total number of observations	145500
Number of reflections	32918
<I/σ(I)>	10.2
Completeness [%]	99.6	98.5	98.2
Redundancy	4.4	3.8	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.5 M Ammonium Sulfate, 100 mM MES