4XUU

The hSac2 domain from human phosphoinositide phosphatase Sac2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	61.772, 82.852, 157.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.620
R-factor	0.2502
Rwork	0.248
R-free	0.28670
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.894
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge		0.047	0.613
Number of reflections	25539
<I/σ(I)>	29
Completeness [%]	99.0	98	100
Redundancy	7.7	7.3	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	300.15	0.2 M ammonium sulfate, 0.1 M Tris-HCl pH 8.0, and 20% PEG3350. Long rod-shaped crystals formed within 2-3 days.