4XU5

Crystal structure of MvINS bound to a bromine-derived 14C Diacylglycerol (DAG) at 2.1A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9185
Spacegroup name	H 3
Unit cell lengths	83.393, 83.393, 124.885
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.689 - 2.100
R-factor	0.2008
Rwork	0.200
R-free	0.22510
RMSD bond length	0.014
RMSD bond angle	1.677
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.100
Number of reflections	37844
<I/σ(I)>	20
Completeness [%]	99.9
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 400