4XTT

Structural Studies of Potassium Transport Protein KtrA Regulator of Conductance of K+ (RCK) C domain in Complex with Cyclic Diadenosine Monophosphate (c-di-AMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	CCD
Collection date	2014-10-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 43 21 2
Unit cell lengths	84.976, 84.976, 85.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.347 - 2.708
R-factor	0.2331
Rwork	0.229
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j91
RMSD bond length	0.005
RMSD bond angle	0.997
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.145	0.057	0.641
Total number of observations	101464
Number of reflections	9002
<I/σ(I)>	6.8
Completeness [%]	99.8	98.5	100
Redundancy	11.3	10.9	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM Bis-Tris pH 6.0, 24.5%(w/v) PEG6000, 250mM Lithium acetate