4XTQ
Crystal structure of a mutant (C20S) of a near-infrared fluorescent protein BphP1-FP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.656, 53.113, 107.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.640 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.20800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.058 | 0.760 |
Number of reflections | 37535 | |
<I/σ(I)> | 10.9 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.1 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 3.2 M NaCl, 0.08 M Tris-HCl, pH 8.0 |