4XTN
Crystal structure of the light-driven sodium pump KR2 in the pentameric red form, pH 4.9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-21 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.969 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.690, 107.000, 240.800 |
Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
Resolution | 48.900 - 2.200 |
R-factor | 0.1968 |
Rwork | 0.195 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xtl |
RMSD bond length | 0.004 |
RMSD bond angle | 0.832 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.900 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.975 | |
Number of reflections | 192342 | |
<I/σ(I)> | 7 | 1.2 |
Completeness [%] | 96.2 | 97.8 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | The crystals were grown using in meso crystallization technique |