Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 92.277, 94.555, 106.390 |
| Unit cell angles | 93.03, 115.06, 102.97 |
Refinement procedure
| Resolution | 19.852 - 2.700 |
| R-factor | 0.1859 |
| Rwork | 0.183 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yzs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.103 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 83703 | |
| <I/σ(I)> | 11.7 | 3.2 |
| Completeness [%] | 97.4 | 97.5 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 20% W/V PEG3350, 0.2M MG NITRATE, 0.3M NACL AND 10MM NH4ACETATE PH 4.6 |
