4XSO
Crystal structure of apo-form Alr3699/HepE from Anabaena sp. strain PCC 7120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.289, 133.469, 142.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.710 - 2.010 |
| R-factor | 0.19711 |
| Rwork | 0.194 |
| R-free | 0.24567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.806 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.084 | 0.481 |
| Number of reflections | 48403 | |
| <I/σ(I)> | 17.56 | 2.96 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 1.0 M ammonium citrate tribasic pH 7.0, 0.1 M Bis-Tris propane pH 7.0 |
