4XSK
Structure of PAItrap, an uPA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 280 |
Detector technology | CCD |
Collection date | 2013-07-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 |
Unit cell lengths | 121.025, 121.025, 42.774 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.940 - 1.500 |
R-factor | 0.1757 |
Rwork | 0.174 |
R-free | 0.20865 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4h42 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.219 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.940 | |
High resolution limit [Å] | 1.500 | |
<I/σ(I)> | 27 | |
Completeness [%] | 99.8 | 99.71 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 2.2 M ammonium sulfate, 5% PEG 400, 50 mM sodium citrate (pH 4.6) |