4XSK
Structure of PAItrap, an uPA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 280 |
| Detector technology | CCD |
| Collection date | 2013-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 |
| Unit cell lengths | 121.025, 121.025, 42.774 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.940 - 1.500 |
| R-factor | 0.1757 |
| Rwork | 0.174 |
| R-free | 0.20865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h42 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.219 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.940 | |
| High resolution limit [Å] | 1.500 | |
| <I/σ(I)> | 27 | |
| Completeness [%] | 99.8 | 99.71 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 2.2 M ammonium sulfate, 5% PEG 400, 50 mM sodium citrate (pH 4.6) |
