4XRK

Crystal Structure of Importin Beta in a Polyethylene Glycol Condition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.77318
Spacegroup name	P 21 2 21
Unit cell lengths	64.410, 121.190, 132.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.642 - 3.250
R-factor	0.2909
Rwork	0.289
R-free	0.31680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xri
RMSD bond length	0.005
RMSD bond angle	1.024
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.642		3.370
High resolution limit [Å]	3.250	50.000	3.250
Rmerge	0.149		0.726
Rmeas	0.168		0.835
Total number of observations	73621
Number of reflections	16105		1518
<I/σ(I)>	8.02		1.65
Completeness [%]	95.0		85.6
Redundancy	4.57		3.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.9	293.15	14 % (w/v) PEG 4000, 8 % (w/v) PEG 200, 10 mM magnesium chloride, 2 % (w/v) benzamidine hydrochloride hydrate, 100 mM Tris pH 8.9