4XRI

Crystal Structure of Importin Beta in an Ammonium Sulfate Condition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.826610
Spacegroup name	P 21 21 21
Unit cell lengths	62.860, 85.040, 207.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.332 - 2.050
R-factor	0.1997
Rwork	0.198
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nd2
RMSD bond length	0.003
RMSD bond angle	0.746
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.330		2.170
High resolution limit [Å]	2.050	50.000	2.050
Rmerge	0.032		0.644
Rmeas	0.038		0.755
Total number of observations	254741
Number of reflections	70347		11272
<I/σ(I)>	19		2.04
Completeness [%]	99.2		99.7
Redundancy	3.6		3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293.15	1 M ammonium sulfate, 5 mM magnesium chloride, 100 mM sodium citrate