4XRD
Salmonella typhimurium AhpC W169F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-01 |
| Detector | FUJI |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 125.870, 171.200, 135.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.705 - 2.300 |
| R-factor | 0.1944 |
| Rwork | 0.192 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ma9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.064 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.900 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 62493 | |
| <I/σ(I)> | 6.5 | 0.5 |
| Completeness [%] | 96.4 | 98.2 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 1.4 M MgSO4, 0.1 M MES, pH 6.5 |
