4XRC

Antibody hemagglutinin Complexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-03
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	248.420, 51.080, 127.610
Unit cell angles	90.00, 99.94, 90.00

Refinement procedure

Resolution	41.918 - 2.740
R-factor	0.1945
Rwork	0.192
R-free	0.24780
RMSD bond length	0.004
RMSD bond angle	0.907
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_1760)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.810
High resolution limit [Å]	2.740	2.740
Rmerge		0.598
Number of reflections	42101
<I/σ(I)>	14.5	2.2
Completeness [%]	99.8	99.8
Redundancy	5.1	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	200 mM ammonium sulfate, 100 mM HEPES pH 7.5, 25% PEG 3350.