4XR1
Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- AG/AT mismatch.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.811, 64.811, 246.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.680 - 2.400 |
| R-factor | 0.20126 |
| Rwork | 0.198 |
| R-free | 0.26899 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i06 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.125 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.711 |
| Number of reflections | 21577 | |
| <I/σ(I)> | 24 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 8-12% (v/v) PEG 3350, 100 mM NaI, 50 mM Bis-Tris |
