4XQS
Crystal structure of human FPPS in complex with one magnesium ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-25 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 110.770, 110.770, 76.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.900 - 2.300 |
| R-factor | 0.19453 |
| Rwork | 0.192 |
| R-free | 0.23248 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2f7m |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.627 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.8.0073) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.900 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 1.035 |
| Number of reflections | 21801 | |
| <I/σ(I)> | 17.3 | 2.2 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 9.6 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295.15 | 1.36 M magnesium sulfate, 15% glycerol, 0.085 M MES |
