4XQO

Crystal structure of hemagglutinin from Jiangxi-Donghu (2013) H10N8 influenza virus in complex with 6'-SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-17
Detector	PSI PILATUS 6M
Wavelength(s)	1.0716
Spacegroup name	P 1 21 1
Unit cell lengths	64.069, 245.424, 71.298
Unit cell angles	90.00, 112.46, 90.00

Refinement procedure

Resolution	46.168 - 2.850
R-factor	0.261
Rwork	0.259
R-free	0.29140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xq5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.850	7.730	2.850
Rmerge	0.145	0.052	0.589
Rmeas	0.182	0.065	0.721
Rpim	0.108	0.038	0.409
Total number of observations	108611
Number of reflections	42408
<I/σ(I)>	7.6
Completeness [%]	89.0	91.3	90.3
Redundancy	2.6	2.7	2.5
CC(1/2)		0.996	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277.15	0.2 M NaSCN, 20% (w/v) PEG 3350