4XQ7
The crystal structure of the OAS-like domain (OLD) of human OASL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-12-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.800, 57.620, 64.790 |
| Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
| Resolution | 35.505 - 1.600 |
| R-factor | 0.1679 |
| Rwork | 0.167 |
| R-free | 0.19260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1px5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.203 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.510 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.755 |
| Number of reflections | 52563 | |
| <I/σ(I)> | 13.97 | 1.82 |
| Completeness [%] | 99.8 | 99.92 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.636 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 mM Tris pH 7.5, 200 mM LiSO4 and 20% (w/v) polyethylene glycol (PEG) 3350. |
