4XQ5

Human-infecting H10N8 influenza virus retains strong preference for avian-type receptors

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 1 21 1
Unit cell lengths	64.069, 242.992, 70.381
Unit cell angles	90.00, 111.26, 90.00

Refinement procedure

Resolution	48.062 - 2.592
R-factor	0.193
Rwork	0.191
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n5j
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.592	7.050	2.592
Rmerge	0.109	0.053	0.453
Rmeas	0.123	0.059	0.517
Rpim	0.056	0.027	0.245
Total number of observations	274246
Number of reflections	60451
<I/σ(I)>	6.7
Completeness [%]	98.6	98	96.8
Redundancy	4.5	4.5	4.2
CC(1/2)		0.997	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277.15	0.2 M NaSCN, 20% (w/v) PEG 3350