4XPZ

Structure of fission yeast RNA polymerase II CTD phosphatase Fcp1-R271A bound to aluminum fluoride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	55.692, 79.989, 89.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.512 - 1.450
R-factor	0.1551
Rwork	0.154
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ef0
RMSD bond length	0.018
RMSD bond angle	1.560
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.500
High resolution limit [Å]	1.450	1.450
Rmerge	0.053	0.622
Number of reflections	70713
<I/σ(I)>	13.8	1.2
Completeness [%]	98.0	100
Redundancy	6.7	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	35% PEG-550MME and 100 mM MES