4XPU
The crystal structure of EndoV from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2014-06-01 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.892, 84.783, 91.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.190 - 2.400 |
| R-factor | 0.19791 |
| Rwork | 0.195 |
| R-free | 0.25512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nsp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.666 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.200 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.157 | 0.669 |
| Number of reflections | 17499 | |
| <I/σ(I)> | 9.5 | 2.5 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 26% PEG 3350, 0.05M sodium fluoride, 0.1M MES pH 6.5 |
