4XPO

Crystal structure of a novel alpha-galactosidase from Pedobacter saltans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	111.869, 111.869, 114.186
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.370 - 2.100
R-factor	0.15514
Rwork	0.153
R-free	0.18709
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.395
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.076	0.423
Number of reflections	42956
<I/σ(I)>	38.4	6.7
Completeness [%]	100.0	100
Redundancy	13.9	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% PEG MME 2000, 100 mM Tris-HCl