4XPO
Crystal structure of a novel alpha-galactosidase from Pedobacter saltans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 111.869, 111.869, 114.186 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.370 - 2.100 |
R-factor | 0.15514 |
Rwork | 0.153 |
R-free | 0.18709 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.395 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.076 | 0.423 |
Number of reflections | 42956 | |
<I/σ(I)> | 38.4 | 6.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.9 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG MME 2000, 100 mM Tris-HCl |