4XP9
X-ray structure of Drosophila dopamine transporter bound to psychostimulant D-amphetamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.348, 140.748, 166.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.720 - 2.800 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m48 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.168 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 55464 |
<I/σ(I)> | 18.1 |
Completeness [%] | 96.6 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 600 (36%), MOPS (0.1M) |