4XOK
Observing the overall rocking motion of a protein in a crystal.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.724, 50.364, 93.458 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.970 - 2.200 |
R-factor | 0.3037 |
Rwork | 0.303 |
R-free | 0.32360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ehv |
RMSD bond length | 0.007 |
RMSD bond angle | 1.358 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.729 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.083 | 0.175 |
Number of reflections | 10207 | |
<I/σ(I)> | 16.04 | |
Completeness [%] | 93.0 | 62 |
Redundancy | 5.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20mg/ml Ubi in 20mM NH4Ac pH4.3, 50mM HEPES pH7.0, 35% PEG 1500, 25mM Zn Acetate |