4XOH
Mechanistic insights into anchorage of the contractile ring from yeast to humans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2013-02-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9870 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.941, 80.941, 314.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.400 - 2.801 |
| R-factor | 0.2366 |
| Rwork | 0.235 |
| R-free | 0.26880 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.669 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.400 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 26437 |
| <I/σ(I)> | 3.2 |
| Completeness [%] | 86.7 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 10% PEG 10000, 100mM MES, 1M LiCl, 2mM DTT, pH 6.0 |
