4XOD
Crystal structure of a FimH*DsG complex from E.coli F18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.310, 35.540, 72.790 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 47.779 - 1.140 |
| R-factor | 0.1428 |
| Rwork | 0.142 |
| R-free | 0.15850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mcy 1qun |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.166 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1621)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.779 | 1.210 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.033 | 0.610 |
| Number of reflections | 87277 | |
| <I/σ(I)> | 16.6 | 1.6 |
| Completeness [%] | 97.4 | 88 |
| Redundancy | 3.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25 % (w/v) PEG 3350, 0.2 M magnesium chloride, 0.1 M BisTris-HCl pH 5.5 |
