4XOA
Crystal structure of a FimH*DsG complex from E.coli K12 in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.530, 77.624, 78.107 |
| Unit cell angles | 101.54, 111.13, 96.29 |
Refinement procedure
| Resolution | 52.674 - 2.541 |
| R-factor | 0.2409 |
| Rwork | 0.240 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mcy 1qun |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1779)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.674 | 2.690 |
| High resolution limit [Å] | 2.541 | 2.541 |
| Rmerge | 0.135 | 0.564 |
| Number of reflections | 35539 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 90.6 | |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.2M Na Malonate, 20%PEG3350 |
