4XO9
Crystal structure of a FimH*DsG complex from E.coli K12 in space group C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.490, 35.620, 72.890 |
| Unit cell angles | 90.00, 105.89, 90.00 |
Refinement procedure
| Resolution | 45.784 - 1.140 |
| R-factor | 0.1252 |
| Rwork | 0.125 |
| R-free | 0.14320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mcy 1qun |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.465 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1621)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.784 | 1.200 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.038 | 0.691 |
| Number of reflections | 85076 | |
| <I/σ(I)> | 17.8 | 2.8 |
| Completeness [%] | 94.5 | 81.5 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | 25 % (w/v) polyethylene glycol (PEG) 3350, 0.2 M magnesium chloride, 0.1 M BisTris-HCl pH 5.5 |
