4XNQ
Antibody hemagglutinin Complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-14 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.00394 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 250.400, 51.178, 127.702 |
| Unit cell angles | 90.00, 100.17, 90.00 |
Refinement procedure
| Resolution | 33.084 - 2.001 |
| R-factor | 0.1917 |
| Rwork | 0.191 |
| R-free | 0.22520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.134 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.670 | |
| Number of reflections | 97220 | |
| <I/σ(I)> | 26.2 | 1.5 |
| Completeness [%] | 96.9 | 53.9 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 200 mM ammonium sulfate, 100 mM HEPES pH 7.5, 25% PEG 3350 |
