4XNM

Antibody Influenza H5 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-09
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	250.939, 51.427, 127.181
Unit cell angles	90.00, 99.83, 90.00

Refinement procedure

Resolution	29.464 - 2.510
R-factor	0.2149
Rwork	0.214
R-free	0.24120
RMSD bond length	0.002
RMSD bond angle	0.659
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1760))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.510	2.510
Rmerge		0.660
Number of reflections	53874
<I/σ(I)>	14.2	1.7
Completeness [%]	97.6	94.3
Redundancy	4.9	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	20% PEG 10,000