4XNH
Crystal structure of yeast N-terminal acetyltransferase NatE (IP6) in complex with a bisubstrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-30 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.475, 114.515, 146.978 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.595 - 2.100 |
R-factor | 0.1713 |
Rwork | 0.168 |
R-free | 0.22770 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.408 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 82940 | |
<I/σ(I)> | 13 | |
Completeness [%] | 99.7 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 14% PEG 400, 0.1 M MES, pH 6.0 |