4XNH

Crystal structure of yeast N-terminal acetyltransferase NatE (IP6) in complex with a bisubstrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	84.475, 114.515, 146.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.595 - 2.100
R-factor	0.1713
Rwork	0.168
R-free	0.22770
RMSD bond length	0.013
RMSD bond angle	1.408
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	82940
<I/σ(I)>	13
Completeness [%]	99.7
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	14% PEG 400, 0.1 M MES, pH 6.0