4XN0
Tailspike protein mutant E372A of E. coli bacteriophage HK620
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 74.342, 74.342, 174.898 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.720 - 1.750 |
R-factor | 0.1533 |
Rwork | 0.151 |
R-free | 0.19280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xm3 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.758 |
Data reduction software | XDS |
Data scaling software | Aimless (0.2.17) |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.720 | 43.720 | 1.780 |
High resolution limit [Å] | 1.750 | 9.090 | 1.750 |
Rmerge | 0.084 | 0.037 | 0.563 |
Rpim | 0.044 | 0.019 | 0.332 |
Total number of observations | 247205 | 1991 | 11607 |
Number of reflections | 57234 | ||
<I/σ(I)> | 9.5 | 22.7 | 1.9 |
Completeness [%] | 99.7 | 98.1 | 99.6 |
Redundancy | 4.3 | 4.1 | 3.8 |
CC(1/2) | 0.998 | 0.997 | 0.767 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris-HCl, 3.5 M Sodiumformiate |