4XMQ
Crystal structure of the sensory domain of the Campylobacter jejuni chemoreceptor Tlp3 (CcmL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.330, 137.510, 49.100 |
| Unit cell angles | 90.00, 94.46, 90.00 |
Refinement procedure
| Resolution | 26.900 - 1.500 |
| R-factor | 0.1407 |
| Rwork | 0.138 |
| R-free | 0.18870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xmr |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.950 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.045 | 0.313 |
| Number of reflections | 75588 | |
| <I/σ(I)> | 10.5 | 2.3 |
| Completeness [%] | 84.9 | 79.3 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG3350, Ammonium Sulfate |
