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Crystal structure of Fab of HIV-1 gp120 V3-specific human monoclonal antibody 2424

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-11-27
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	69.592, 45.043, 74.778
Unit cell angles	90.00, 96.75, 90.00

Refinement procedure

Resolution	47.862 - 2.680
R-factor	0.2039
Rwork	0.200
R-free	0.28500
RMSD bond length	0.009
RMSD bond angle	1.225
Data scaling software	XDS
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.840
High resolution limit [Å]	2.680	2.680
Number of reflections	13122
<I/σ(I)>	11.5	1.98
Completeness [%]	99.1	98.6
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	296	PEG 8000, Tris, glycerol