4XM4

Structure of Chaetomium Mex67:Mtr2 subunits

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	43.754, 96.087, 194.764
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.043 - 2.950
R-factor	0.2292
Rwork	0.227
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.710
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.043	3.055
High resolution limit [Å]	2.950	2.950
Rmerge	0.092	0.810
Number of reflections	17888
<I/σ(I)>	11.2	1.6
Completeness [%]	99.3	100
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	See manuscript for details