4XLT

Crystal structure of response regulator receiver protein from Dyadobacter fermentans DSM 18053

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97935
Spacegroup name	P 63
Unit cell lengths	62.945, 62.945, 69.486
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.298 - 2.300
R-factor	0.1959
Rwork	0.193
R-free	0.24870
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.627
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.320
High resolution limit [Å]	2.300	7.860	2.300
Rmerge	0.058	0.050	0.665
Rmeas	0.061	0.054	0.706
Rpim	0.019	0.018	0.233
Total number of observations	74878
Number of reflections	6764
<I/σ(I)>	13.9
Completeness [%]	97.6	93.5	97.3
Redundancy	10.8	9.3	8.5
CC(1/2)		0.996	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	297	0.1 M Sodium Acetate, 0.1M HEPES, 22% PEG4000