4XKO
N-terminal domain of Hsp90 from Dictyostelium discoideum in hexagonal form with PEG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97625 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 158.190, 158.190, 46.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.780 - 2.455 |
R-factor | 0.2185 |
Rwork | 0.216 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xcj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.057 |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.780 | 2.590 |
High resolution limit [Å] | 2.455 | 2.455 |
Rmerge | 0.072 | 0.741 |
Number of reflections | 12933 | |
<I/σ(I)> | 24.2 | 4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.2 | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 0.1M Tris, Ammonium sulphate, PEG 200 |