4XKE
Crystal structure of hemagglutinin from Taiwan (2013) H6N1 influenza virus in complex with 3'-SLN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 185.596, 99.253, 133.418 |
Unit cell angles | 90.00, 126.30, 90.00 |
Refinement procedure
Resolution | 49.505 - 2.356 |
R-factor | 0.2317 |
Rwork | 0.230 |
R-free | 0.27230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xkd |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
Rmerge | 0.097 | 0.057 | 0.601 |
Rmeas | 0.117 | 0.069 | 0.751 |
Rpim | 0.065 | 0.038 | 0.444 |
Total number of observations | 238063 | ||
Number of reflections | 78465 | ||
<I/σ(I)> | 7.2 | ||
Completeness [%] | 98.2 | 98.7 | 92.7 |
Redundancy | 3 | 3.2 | 2.3 |
CC(1/2) | 0.994 | 0.736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 10mM NiCl2, 0.1M Tris pH 8.5, 20% (w/v) MPEG 2000 and 20% glycerol |