4XJX
STRUCTURE OF MUTANT (E165H) OF THE HSDR SUBUNIT OF THE ECOR124I RESTRICTION ENZYME IN COMPLEX WITH ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.221, 124.542, 128.540 |
Unit cell angles | 90.00, 107.77, 90.00 |
Refinement procedure
Resolution | 49.090 - 2.400 |
R-factor | 0.2099 |
Rwork | 0.208 |
R-free | 0.24350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w00 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.5) |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.090 | 2.540 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.123 | 0.800 |
Number of reflections | 99544 | |
<I/σ(I)> | 9.37 | 1.65 |
Completeness [%] | 99.2 | 96.3 |
Redundancy | 4.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 278 | 5 ul protein solution were mixed with 2 ul reservoir solution containing 13% (w/v) PEG 4000, 22% glycerol, 0.08 M MES, 2mM DDT, and 0.7 ul of 0.1 M sodium bromide as additive |