4XJI
X-ray structure of LysozymeS2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.710, 78.710, 37.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.355 - 2.000 |
| R-factor | 0.1667 |
| Rwork | 0.164 |
| R-free | 0.20990 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.018 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 11065 | |
| <I/σ(I)> | 48 | 17 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 65 | 44.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.5-1M NaCl, 50-100 mM CH3COONa, pH4.5, 15-30% PEG 400 |
