4XJH
X-ray structure of LysozymeS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-02-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.100, 79.100, 38.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.550 - 2.000 |
| R-factor | 0.1592 |
| Rwork | 0.157 |
| R-free | 0.20450 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.018 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Number of reflections | 8638 | |
| <I/σ(I)> | 45.01 | 15.1 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 102.5 | 49.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.5-1M NaCl, 50-100 mM CH3COONa, pH4.5, 15-30% PEG 400 |
