4XJG
X-ray structure of Lysozyme B2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92049 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.390, 78.390, 37.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.195 - 1.800 |
| R-factor | 0.2017 |
| Rwork | 0.199 |
| R-free | 0.24340 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.014 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 11423 | |
| <I/σ(I)> | 12.8 | 2.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 49.7 | 43.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 1M NaBr, 50-100 mM CH3COONa, pH4.5, 15-30% pEG 400 |
