4XJF
X-ray structure of Lysozyme B1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2014-02-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92049 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.100, 79.100, 38.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.550 - 1.800 |
R-factor | 0.1774 |
Rwork | 0.176 |
R-free | 0.20680 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.089 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 11797 | |
<I/σ(I)> | 12.9 | 3.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 32.3 | 33.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 1M NaBr, 50-100 mM CH3COONa, pH4.5, 15-30% pEG 400 |