4XJ6

Crystal structure of Escherichia coli DncV 3'-deoxy GTP bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	136.070, 46.300, 62.560
Unit cell angles	90.00, 110.61, 90.00

Refinement procedure

Resolution	37.129 - 2.310
R-factor	0.1913
Rwork	0.189
R-free	0.23350
RMSD bond length	0.003
RMSD bond angle	0.669
Data reduction software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.310
Number of reflections	16217
<I/σ(I)>	11.6
Completeness [%]	99.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	PEG3350, sodium chloride, Bis-Tris propane