4XJ2
FerA - NADH:FMN oxidoreductase from Paracoccus denitrificans in complex with FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 43.920, 43.920, 154.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20200 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.062 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 16671 |
| <I/σ(I)> | 21.3915 |
| Completeness [%] | 99.0 |
| Redundancy | 4.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 4000, ammonium sulfate, ammonium acetate, MES |
