4XIZ
Structure of a phospholipid trafficking complex with substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.022, 74.123, 87.912 |
| Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
| Resolution | 34.130 - 2.000 |
| R-factor | 0.2018 |
| Rwork | 0.196 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.116 | 0.082 | 0.375 |
| Total number of observations | 150394 | ||
| Number of reflections | 36740 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 99.4 | 96 | 99.9 |
| Redundancy | 4.1 | 3.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 2%(v/v)Tacsimate pH 6.0, 0.1M Bis-Tris pH 6.5, 20%(w/v) PEG3350 |
