4XIS
A METAL-MEDIATED HYDRIDE SHIFT MECHANISM FOR XYLOSE ISOMERASE BASED ON THE 1.6 ANGSTROMS STREPTOMYCES RUBIGINOSUS STRUCTURES WITH XYLITOL AND D-XYLOSE
Experimental procedure
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 94.640, 99.970, 103.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.135 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.031 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.530 * |
| Total number of observations | 379659 * |
| Number of reflections | 70624 * |
| Completeness [%] | 98.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 8.0 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 21 (%sat) | |
| 3 | 1 | drop | 0.5 (mM) | ||
| 4 | 1 | drop | HEPES | 50 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 43 (%sat) | |
| 6 | 1 | reservoir | HEPES | 50 (mM) |
