4XIG

Crystal structure of bacterial alginate ABC transporter determined through humid air and glue-coating method

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	72.175, 133.412, 272.687
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.559 - 3.402
R-factor	0.2383
Rwork	0.236
R-free	0.28950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.820
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.460
High resolution limit [Å]	3.400	3.400
Rmerge		0.536
Number of reflections	37120
<I/σ(I)>	12.7
Completeness [%]	99.8	100
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG3000, N-(2-acetamido)iminodiacetic acid, sodium chrolide, 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxypropanesulfonate